Are you still worried about choosing an organic phase or an aqueous phase?
Now the editor has sorted out the detailed selection cheats, come and take a look!
Syringe Filter Brief Introduction
In HPLC and GC analysis, impurities in samples and solvents not only easily cause damage to liquid phase pumps, injection valves, chromatographic columns, etc., but also often affect the analysis results. Therefore, filtering the sample and solvent in advance can not only effectively reduce the wear of the pump, and prevent the blockage of the injection valve, pipeline, and chromatographic column, but also greatly reduce the interference of impurities on the chromatogram, thereby improving the sensitivity and accuracy.
• Various membrane materials
• Filtration of samples and solvents for HPLC, GC preparation
• Standard Luer fittings
• Ultra-clean polypropylene housing, low extractable, suitable for trace analysis
• Low residual volume
• Economical and environmentally friendly
Syringe Filter Specifications Selection Guide
The selection of the size of the syringe filter is usually related to the volume of the sample: Usually, when the sample volume is less than 1mL, a 4mm diameter syringe filter is used; When the sample volume is 1-10mL, a 13mm diameter syringe filter is used; The sample volume is 10 -100mL, use a 25mm diameter syringe filter; When the sample volume is 10-200mL, choose a 33mm diameter syringe filter.
Syringe Filter Media Selection Guide
1. Cellulose Acetate (CA)
The hydrophilic filter membrane has uniform pore size, high porosity, low resistance, a fast filtration rate, and minimal adsorption. They are mainly used to filter out particles and bacteria in biological samples and water-soluble samples.
2. Nylon (NY)
Universal filter membrane, suitable for the filtration of most sample solutions and solvents. The filter membrane itself has hydrophobic properties, but it is also suitable for the filtration of aqueous solutions and organic solvents, so it is often used for HPLC and GC sample and solvent filtration.
3. Polyvinylidene fluoride (PVDF)
A hydrophobic membrane, with extremely low protein adsorption, is especially suitable for filtration and sterilization of biological samples, with low resistance and fast filtration. In addition, it has high chemical compatibility and is suitable for the filtration of most media.
4. Polyethersulfone (PES)
Hydrophilic membrane, low resistance, fast filtration, low protein adsorption, especially suitable for the filtration of viscous biological and cell tissue samples. In addition, it has good permeability and is suitable for the fast filtration of large volumes of aqueous samples.
5. Polytetrafluoroethylene (PTFE)
Hydrophobic membrane, suitable for the filtration of organic solvent media samples (including strong acids, and strong bases) and organic solvents. Although the filter itself is hydrophobic, it can also be made hydrophilic by adding ethanol and deionized water.
6. Glass Fiber (GF)
Universal pre-filter, 1.0 μm pore size, suitable for filtration of high particle content or viscous samples; 2-4 times higher throughput than syringe filters without GF pre-filter.
7. Mixed Cellulose Esters (MCE)
The pharmaceutical industry needs autoclaved water needles, large infusions to filter out particles; For sterilization of heat-sensitive drugs (biochemical preparations such as insulin ATP, coenzyme A, etc.), use a 0.45 μm filter (or 0.22); Analysis and determination of particulates and oil incompatibles in solution, and determination of water pollution index; It is also used in scientific research departments such as somatic cell hybridization and mitochondrial complementation prediction of hybrid vigor.